Effects of the continuous nursing education on anxiety level of caregivers of patients with all-robot coronary artery bypass surgery / 四川精神卫生

BackgroundMost of the researches on continuous nursing education is from the perspective of patients， and there is a lack of studies on the impact of continuous nursing education on patient caregivers， and the care quality of caregivers is closely related to postoperative rehabilitation status of the patients. ObjectiveTo investigate the effect of continuous nursing education on anxiety relief of caregivers of patients with all-robot coronary artery bypass surgery， and to provide references for improving caregivers' anxiety and promoting patients' recovery. MethodsA total of 120 caregivers of patients with all-robot coronary artery bypass surgery at the First and Sixth Center of 301 Chinese PLA General Hospital were included from January 1， 2021 to December 31， 2022. The caregivers were randomly divided into study group and control group with 60 cases in each group by systematic random sampling method. Before the patient discharge from the hospital， all caregivers received routine nursing education. The study group received continuous nursing education for 4 weeks after patient discharged. Self-rating Anxiety Scale （SAS） was used to evaluate the two groups before and one month after discharge. ResultsOne month after discharge， SAS score in the study group was significantly lower than that in the control group ［（57.77±14.08） vs. （70.19±13.60）， t=-4.913， P<0.01］， and the proportion of SAS score above 60 in the study group was significantly lower than that in the control group （41.67% vs. 75.00%， χ2=-13.714， P<0.01）. ConclusionContinuous nursing education may help reduce the level of anxiety in caregivers of patients with all-robot coronary artery bypass surgery.

The relationship and clinical significance of TMS1,MBD2 and DNMT1 in hepatocellular carcinoma / 国际检验医学杂志

Objective To observe the expression of methylation related silent target 1 (TMS1),DNA methyltransferase 1 (DNM T1)and methyl CpG binding domain protein 2 (MBD2),and the methylation of the TMS1 gene promoter,in hepatocellular carcinoma (HCC),and to investigate the relationship between the three genes and the clinical diagnostic significance.Methods The protein expression of TMS1,MBD2 and DNMT1 in 48 cases of HCC tissues and 48 case of normal tissues were detected by immunohistochemistry (IHC);the methylation status of TMS1 gene in serum of 34 patients with HCC,26 patients with hepatitis B and 23 healthy controls were analyzed by methylation specific polymerase chain reaction (MSP).Results The posi-tive rates of TMS1 expression in HCC tissues and normal tissues were 26.08% and 97.92%,respectively;the positive rate of MBD2 expression were 18.75% and 80.00%,respectively;the positive rate of expression were 77.08 % and 32.25%,respectively.The positive rates of TMS1 and MBD2 expression in HCC tissues were significantly lower than those in the normal tissues,and DNM T1 expression was higer than that in the normal tissues,and the difference was statistically significant (P<0.05).The expression TMS1 and MBD2 were neg-atively correlated with DNMT1 (P< 0.05).TMS1 protein expression was associated with TNM stage and tumor differentiation but not associated with the age and gender (P<0.05).The methylation detection rates of TMS1 gene in HCC group,hepatitis B group and healthy group were 70.6%,50.0% and 0.0%,respective-ly,and the differences were statistically significant (P<0.05).The methylation detection rate of HCC group and hepatitis b group was obviously higher than the normal group,which aggravated with the increase of tumor grade and stage.Conclusion TMS1 hypermethylation may be an early event in the carcinogenesis of liver tissue,the abnormal expression of TMS1,MBD2 and DNM T1 proteins plays an important role in the oc-currence and the development of HCC,w hich can be used as new molecular markers for the early diagnosis and prognosis of HCC,and become the new targets for the treatment of HCC.

Cloning and expression of a hemolysin gene of Aeromonas hydrophila and the immunogenicity of the toxoid / 生物工程学报

According to the GenBank sequences (GenBank Accession No. AF539467), one pair of primers was designed to amplify hly gene of Aeromonas hydrophila by PCR. After sequencing, homology analysis indicated that a DNA fragment of 1485 bp was amplified from isolated DNA from Aeromonas hydrophila, and it shared more than 99% homology in nucleotide sequence compared with other reference strains in GenBank. The gene was cloned in pET-28a vector to construct a recombinant plasmid pET-28a-hly, which was transformed into Escherichia coli BL21 (DE3), and the recombinant strain BL21(DE3)(pET-28a-hly) was obtained. The hemolysin was highly expressed when the recombinant strain BL21 (DE3) (pET-28a-hly) was induced by IPTG. The expressed protein was 56 kD as estimated by 15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The immunogenicity of the expressed Hly protein was confirmed by Western blotting. Mice were immunized with inactivated whole bacteria vaccine and the genetic engineering vaccines showing promise that all these vaccines have a high protective ability. The results showed that the recombinant strain BL21 (DE3)(pET-28a-hly) could be candidate of hemolysin toxoid vaccine to provide protective immunity against diseases caused by Aeromonas hydrophila.

Inhibition of glucosylceramide synthase expression and reversal of multidrug resistance in human breast cancer cells by shRNA / 基础医学与临床

Objective To construct the plasmid containing short hairpin RNA (shRNA) of GCS in order to suppress the expression of GCS and to reverse the multidrug resistance in drug-resistant breast cancer cells. Methods Two reverse repeated motifs of sequence targeting GCS with 6 bp spacer were designed and synthesized in vitro,then they were inserted into the plasmid pSUPER. The recombinant plasmids were transfected into human breast cancer cells. GCS expression was detected by Western blot and immunocytochemistry. caspase-3 expression and its activity were assessed using Western blot and colorimetry,flow cytometry was performed to analyze the ratio of apoptosis. Results MTT method was used to evaluate the 50% inhibition concentration. Enzyme digestion analysis and DNA sequencing confirmed that the recombinant plasmids were successfully constructed. GCS protein content decreased 80%,82% respectively after transfection with recombinant plasmids. The positive rate of GCS expression reduced to 18.1%,16.7% respectively. caspase-3 expression and its activity were significantly higherand the apoptosis of cells increased dramatically. The drug resistance of breast cancer cells to antineoplastic drugs declined significantly. Conclusion shRNA can suppress GCS expression in human drug-resistant breast cancer cells effectively and restore the sensitivity to several antineoplastic drugs.

Grape seed polyphenols reverses multidrug resistance of human breast cancer / 中国普通外科杂志

Objective To explore if grape seed polyphenols (GSP) reverses human breast cancer multidrug resistance. MethodsIn this study,adriamycin-resistant human breast carcinoma cells ( MCF-7/ADR ) and its parental cells (MCF-7) were used to determine the effect of GSP. MTT assay was adopted to evaluate the cytotoxity. Western blot and Northern blot were performed to observe the expression of MDR1 in MCF-7/ADR. Adriamycin accumulation was measured by flow cytometry (FCM). ResultsGSP ( 1.2 mg/L ,2.4 mg/L) inhibited the expression of P-gp to 80.83%( t =5.58,P